The US DOE Joint Genome Institute (JGI) is a high throughput sequencing and genomic research center involved in a myriad of sequencing projects. JGI’s major effort is the sequencing of genomes and transcriptomes of plants, microbes and environmental metagenomic samples of relevance to the DOE missions of carbon sequestration, bioremediation and energy production. Roche/454’s platform utilizes emulsion PCR for template amplification and pyrosequencing technology on high well-density...